全文获取类型
收费全文 | 1407篇 |
免费 | 91篇 |
国内免费 | 83篇 |
出版年
2023年 | 20篇 |
2022年 | 39篇 |
2021年 | 32篇 |
2020年 | 23篇 |
2019年 | 40篇 |
2018年 | 44篇 |
2017年 | 30篇 |
2016年 | 31篇 |
2015年 | 52篇 |
2014年 | 120篇 |
2013年 | 137篇 |
2012年 | 88篇 |
2011年 | 82篇 |
2010年 | 50篇 |
2009年 | 40篇 |
2008年 | 42篇 |
2007年 | 65篇 |
2006年 | 42篇 |
2005年 | 44篇 |
2004年 | 22篇 |
2003年 | 35篇 |
2002年 | 27篇 |
2001年 | 40篇 |
2000年 | 36篇 |
1999年 | 32篇 |
1998年 | 26篇 |
1997年 | 33篇 |
1996年 | 25篇 |
1995年 | 35篇 |
1994年 | 36篇 |
1993年 | 31篇 |
1992年 | 22篇 |
1991年 | 19篇 |
1990年 | 18篇 |
1989年 | 8篇 |
1988年 | 12篇 |
1986年 | 13篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 8篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1976年 | 8篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1972年 | 5篇 |
排序方式: 共有1581条查询结果,搜索用时 156 毫秒
21.
初选藤仓赤霉菌(Gibberella fujikuroi)农大17菌株为赤霉素A_4、A_7(GA_4、GA_7)的生产菌,该菌株GA_(4+7)的积累量,除与营养条件有关外,温度和pH是极为重要的因子,随着发酵温度从28℃上升至32℃,GA_(4+7)的产量由21μg/ml增至81μg/ml,GA_3由702μg/ml降至328μg/ml。pH回调至中性,GA_(4+7)的产量由75μg/ml增至180μg/ml,GA_3由322μg/ml降至211μg/ml。此外,设法延长发酵周期也是增加GA_(4+7)的一个因素。综合上述条件,即发酵过程中,发酵液的pH由48h前的4回调并维持在6.7左右,温度由28℃上调并控制在32℃,摇瓶培养12天,GA_(4+7)的产量达890μg/ml,20—600L发酵罐发酵240h,GA_(4+7)产量达680μg/ml左右。GA_(4+7)浓度的测定亦作了简化处理,发酵液不经提取,可直接用硅胶板G薄层层析(TLC)后进行荧光比色,产品测定宜采用高效液相色谱法(HPLC)。按照上述条件培养的农大17菌株,产生GA_3、GA_7和GA_4的比例为23.131:16.105:31.258,GA_4高于有关报道。 相似文献
22.
兔出血症病毒主要结构多肽的氨基酸分析 总被引:1,自引:0,他引:1
兔出血症病毒主要结构多肽的氨基酸分析王恒安,杜念兴,徐为燕(南京农业大学,南京210095)关键词兔出血症病毒,结构多肽,氨基酸分析有关兔出血症病毒(RHDV)结构多肽的报道很多,有认为只有1条,有报道4条的,也有报道多达6条的。但其主要结构多肽为分... 相似文献
23.
浒苔多糖的分离、纯化和分析 总被引:1,自引:0,他引:1
浒苔(Enteromorphaprolifera)经热水提取,Sevage法除去蛋白质,用乙醇沉淀,SephadexG-100柱层析,得浒苔多糖(简称EP)精制品。经SephadexG-200柱层析鉴定为单一对称性洗脱峰。红外光谱分析具有多糖特征吸收峰,紫外光谱分析未见有核酸和蛋白质的特征吸收峰。总糖含量为88.8%,其中糖醛酸含量为33.6%。单糖组成为L-阿拉伯糖、L-岩藻糖、D-甘露糖、D-半乳糖及D-葡萄糖,平均分子量为25000。 相似文献
24.
Michael W. Palmer 《植被学杂志》1995,6(3):447-447
Abstract. This note is to apologize for an error in the computer program used to evaluate the random data used in Fuzzy Set Ordination according to Zhang & Oxley. After correction of this error no artifacts could be detected any longer. However, the basic conclusion of the earlier critical note still stands: if one selects environmental variables after analyzing the results of a multivariate gradient analysis, and then uses these variables as input into a multiple univariate gradient analysis, the results are expected to be comparable. 相似文献
25.
Summary The Upper Santonian to Lower CampanianLacazina Limestone consists of massive, often amalgamated beds of packstones and grainstones which were deposited in a shallow marine
environment. The most abundant skeletal components are miliolid foraminifera, echinoderm, bivalve and bryozoan fragments,
peloids and sparse red algae. Small, solitary corals only occur sporadically. Hermatypic corals, sponges and green algae are
missing. The series which reaches thicknesses between 60 m and 160 m, was sampled at intervals of 0.5 m at five localities.
The petrographic features throughout the series are mainly a product of changing depositional energy. The limestones are well
cemented. Diagenesis is characterized by a transition from marine phreatic to burial cementation. Syntaxial and blocky calcite
cements predominate over early acicular to bladed and microgranular cements.
The faunal association within theLacazina Limestone exhibits features typical for temperate water i.e.foramol carbonates. On the other hand, oxygen (δ18O =-1.7 to −6.3 ‰ PDB) and carbon (δ13C to 3.0‰ PDB) isotope values of diagentically unaltered bivalve shells indicate warm surface waters corresponding better
to the palaeogeographical situation of theLacazina Limestone. Nutrient-surplus is proposed as a limiting factor preventing the development of reefs and finally ofchlorozoan sediments.
In the sense of sequence stratigraphy, theLacazina Limestone is interpreted to contain transgressive and highstand systems tracts. This interpretation fits well into theHaq-Vail-curve. The series shows no visible high-frequency cyclicity in the field. Several cycles were found by means of principle
component analysis and spectral analysis. Their relationships to the Milankovitch spectrum are discussed.
The ammonite fauna of the unit and of preceding sediments (late Coniacian to early Campanian) is described and some inoceramids
are figured. They permit—for the first time—the exact dating of theLacazina Limestone in the Basco-Cantabrian Basin (BCB) and throw light on a prominent faunal change at the Coniacian/Santonian boundary.
The Cenomanian to Coniacian ammonite faunas which were dominated by endemic Tethyan pseudoceratitic faunas are replaced by
cosmopolitan species dominated by Madagascan elements. This drastic change permits speculations about the installation of
a new oceanic current system in the Santonian. 相似文献
26.
The seasonal dynamics of epilithic algae in a third order pristine forest stream were analyzed over a period of 2 years. Stream water was slightly acidic and nutrient poor. Encrusting, filamentous flocs, and filaments were found. Algal standing crop was high (mean concentration of Chl a 16–43 mg m–2) in spring. Filamentous algae contributed most to standing crop. Diatoms made up over 85% by number of the epilithon. Blue-greens were abundant upstream, and chlorophytes downstream. This shift was ascribed to greater light availability downstream. The community was more diverse during spring. Water current was the most important variable regulating epilithon structure. Total phosphorus (TP), orthophosphate (O-PO
inf4
su3–
), silica (Si4+), nitrate (NO
inf3
su–
) and conductance correlated negatively with flow rate. Green algae showed a positive correlation to phosphorus during low and stable flow. During rapid runoff, diatoms were the most resistant forms. Seasonal change in the epilithic community was mainly regulated by fluctuations in flow rate. 相似文献
27.
Three protocols for the determination of inorganic and organic sulfur fractions were tested for their suitability to estimate total indigenous organic sulfur (Sorg) and35Sorg formed from added35SO4
2– in sediments of chemically dilute lakes in the ELA. The protocols tested have all been reported in the literature. It was found that two protocols involving sequential analyses for S fractions following acid treatment gave estimates of both Sorg and35Sorg up to 87% lower than a non-sequential protocol. The low estimates were largely due to hydrolysis and solubilization of solid phase S which was then removed in a rinsing step. The non-sequential protocol, in which total reduced inorganic sulfur and total sulfur were determined on separate aliquots, is recommended as the most reliable of the three. Individual analyses in this protocol were verified for these lake sediments using a variety of S standards. 相似文献
28.
Dr. Andrei V. Dronov 《Facies》1993,28(1):169-180
Summary Several Waulsortian-type mud mounds nearly 500 m thick and about 5 km long occur in the Middle Paleozoic carbonate section
of the Aktur nappe in the mountains on the right bank of Isfara river. These buildups form a well developed barrier system
that stretches along the South Ferganian carbonate platform margin and divides the carbonate complex into a fore-reef and
a back-reef part. The time of the mounds' most active growth was from the Late Silurian (Ludlow) to the Middle Devonian (Eifel).
Three main facies types can be recognized in the mud mounds: 1. micritic core facies, 2. sparitic flank facies and 3. loferitic
capping facies. The central massive or crudely bedded part of the mounds consists of white or light grey clotted micrite.
Macrofossils are rare. The sparitic flank facies in contrast consists of coarse and densely packed crinoidal wackestone-floatstones
with some brachiopod shell debris. Solitary rugose corals, tabulate corals, stromato-poroids and fragments of mollusks are
also abundant. The tops of the mounds are usually covered with loferitic pelmicrites or oolitic grainstone caps. Stromatactis-like
structures are very rare and poorly developed in the South Ferganian mud mounds. However, almostin all such mounds horizons
of calcitic breccias can be found.
In order to explain all the features found in the Fergana mounds an ‘atoll-like’ model has been proposed which starts the
evolution of the mud mounds with a small nucleus bioherm. The main stage of the evolution corresponds to an atoll-like structure
developing on the surface of shallow water platforms. White clotted micrite of the mound core facies is interpreted as a accumulation
of fine-grained sediment in an inner lagoon flanked by crinoidal bar deposits. The mound flank facies represents the atoll
rim deposits from where the carbonate mud is derived. The capping loferitic facies is considered as tidal flat deposit that
developed on top of the buildups during the last stage of its evolution. The knoll shape of the mounds is explained by the
retreat of the atoll flanking crinoidal bars back into the inner lagoon during the rise in sea level. Stromatactis-like structures
of small cavities filled with sparry calcite owe their existence to burrowing organisms. Calcitic breccias are interpreted
as paleokarst collapse breccias. They indicate that the tops of the mud mound became subaerially exposed. Other evidence for
a subaerial exposure can be seen in the occurrence of Variscian ‘black and white’ limestone gravel on the tops of some mud
mounds. According toWard et al. (1970) these sediments were produced above the sea level at the edge of hypersaline lakes situated on islands. 相似文献
29.
应用于预生物合成研究的核酸碱基分析方法何裕建,戚生初(中国科学院生物物理研究所,北京10O101)(北京大学技术物理系,北京10O871)关键词HPLC分析;核酸碱基;离子交换色谱在生命起源研究中,核酸大分子起源是化学进化的一个重要课题,众所周知,核... 相似文献
30.
Logistic曲线参数的一个最佳估计方法 总被引:3,自引:0,他引:3
本文提出用O.628优选法和SAS/STAT软件NLIN模块中的DUD法,对Logistic曲线中的参数k,a和b可得到最佳估计。 相似文献